The Blockbuster store in Barnsley town centre is to close after administrators for the firm confirmed it was shutting a further 164 rental outlets nation-wide.
Eight staff at the Peel Street store are now facing redundancy but it will remain open for business as usual. Customers will be notified in advance of closure so they can return outstanding rental items.
Another store on Station Road in Wombwell has escaped closure and will continue to trade as normal while administrators Deloitte negotiate with bidders.
A spokesman for Deloitte said there will be a number of closing down promotions across stores to supplement those already on offer across the estate.
Lee Manning, joint administrator, said: "We have continued to review the performance of individual stores since our appointment a month ago and have concluded that further closures are necessary in order to restructure the company for sale."